Figure 5.
SDS-PAGE analysis of mouse and human PPT transfected in COS-1 cells and immunoprecipitated with anti-human PPT antibody. (A) Cells transfected with mouse PPT construct, pulse labeled for 1 hr and chased at 2, 5, and 24 hr. Both cells and media were immunoprecipitated. As a control cells transfected with a human PPT construct are shown atright. (B) Maturation of the intracellular mouse and human PPT polypeptides. One-hour pulse and 0-, 2-, and 5-hr chase. (C) Glycosidase digestions of the transfected cells after 20-min pulse, 1-hr chase, and immunoprecipitation. (−) Untreated; (E) Endo H; (P) PNGase F; (T) tunicamycin.
