Vector–Hexamer PCR Isolation of All Insert Ends from a YAC Contig of the Mouse Igh Locus

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Figure 2.Figure 2.Figure 2.Figure 2.
Figure 2.

Vector–hexamer PCR products. (a) Products of the initial PCR using the outermost YAC vector right arm primer R-I with thePvuII primer. Only nonspecific products are visible. (b) Products of the second round of PCR using the first nested right arm primer R-II with the PvuII primer. Both nonspecific and a few specific products are visible. (c) Products of the third round using the second nested right arm primer R-III with thePvuII primer. Bright YAC-specific products are apparent in many lanes. (d) Third-round PCR products from several different YACs made with the left arm L-III primer in conjunction with three different hexamer primers. The center gel demonstrates a background band amplified from the yeast host near the position of the largest size marker band (φX174 digested with HincII) in all four YACs and the control YAC.

This Article

  1. Genome Res. 8: 673-681

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