Rescue of Targeted Regions of Mammalian Chromosomes by in Vivo Recombination in Yeast

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Figure 5.
Figure 5.

Comparison of the YACs with genomic DNA. DNA was isolated from Trp+yeast transformants and chicken cells containing the targeted human chromosome 11, digested by EcoRI or BglII, and hybridized to the 5′ external probe shown in Fig. 2. (Lanes1,5) Parental DT40 11-3 line. (Lanes 2,6) DT40 11-3 cells carrying the targeted human chromosome 11. (Lanes3,4,7,8) Two different YAC isolates. In prediction that the YACs contain genomic sequences flanking the targeting vector, digestion identifies identical bands in YACs and genomic DNA.

This Article

  1. Genome Res. 8: 666-672

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