(A) YAC contig covering the 6p21.2–21.3 region. YACs from three libraries are listed to the left and markers at thetop. The YACs and markers in 6p21.1 are shown ordered toward the centromere. Vertical bars indicate the presence of markers in the particular clone and thin interrupted lines indicate chimeric parts. An asterisk indicates that the clone has been used to isolate the cosmids shown in B and Fig. 4. The shaded bar under theZNF76–D6S439 region indicates the finely mapped segment shown in B. (B) Restriction map of the 350-kbZNF76–TCP-11 region. The positions of the two genes and D6S439 are shown above, and the position of MluI andBssHII sites as determined by PFGE analysis of YAC 9.2 and digestion of cosmids are below. Cosmids are derived from the chromosome 6 library or by subcloning the YAC 9.2. The left end of the YAC is chimeric to 6p12 (hatched bar) and the same was found for the subcloned cosmid 4 that hybridizes by FISH to 6p21 and 6p21.2. The region deleted in YAC 9.2 is indicated by a gap in cosmids 43,23R/2,11R, 28R, and 21 (see also Fig. 3). The EcoRI map derived from the cosmids and the position of the repeated elements with relative orientation are presented at the bottom.
