Physical Map of Human 6p21.2–6p21.3: Region Flanking the Centromeric End of the Major Histocompatibility Complex
- Nicos Tripodis1,
- Ruth Mason1,
- Sean J. Humphray2,
- Angela F. Davies1,
- Jethro A. Herberg3,
- John Trowsdale3,6,
- Dean Nizetic4,
- Gabriele Senger5, and
- Jiannis Ragoussis1,7
- 1Division of Medical and Molecular Genetics, United Medical and Dental School of Guy’s and St. Thomas’, Guy’s Hospital, London SE1 9RT, UK; 2The Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK; 3Imperial Cancer Research Foundation (ICRF) Laboratories, London WC2A 3PX, UK; 4Centre for Applied Molecular Biology, University of London, School of Pharmacy, London WC1N 1AX, UK; 5Institut für Humangenetik und Anthropologie, D-07740 Jena, Germany; 6Department of Pathology, University of Cambridge, Cambridge CB2 1QP, UK
Abstract
We have physically mapped and cloned a 2.5-Mb chromosomal segment flanking the centromeric end of the major histocompatibility complex (MHC). We characterized in detail 27 YACs, 144 cosmids, 51 PACs, and 5 BACs, which will facilitate the complete genomic sequencing of this region of chromosome 6. The contig contains the genes encoding CSBP, p21, HSU09564 serine kinase, ZNF76, TCP-11, RPS10, HMGI(Y), BAK, and the human homolog of Tctex-7 (HSET). The GLO1 gene was mapped further centromeric in the 6p21.2–6p21.1 region towardTCTE-1. The gene order of the GLO1–HMGI(Y) segment in respect to the centromere is similar to the gene order in the mouset-chromosome distal inversion, indicating that there is conservation in gene content but not gene order between humans and mice in this region. The close linkage of the BAK and CSBPgenes to the MHC is of interest because of their possible involvement in autoimmune disease.
Footnotes
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↵7 Corresponding author.
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E-MAIL i.ragoussis{at}umds.ac.uk; FAX 44-171-955-4444.
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- Received July 15, 1997.
- Accepted April 13, 1998.
- Cold Spring Harbor Laboratory Press
