Short-Insert Libraries as a Method of Problem Solving in Genome Sequencing

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Figure 2.Figure 2.
Figure 2.

179I15–H. sapiens chromosome 13 BRCA2 region. (A) After finishing, 179I15 contained a region of 11 bp within a CpG island in which the sequence was unreadable using standard dye primer or dye terminator sequencing. (B) An example of a reverse direction dye primer terminator sequencing reaction over the region (read no. 3284); the sequence obtained from a small insert clone across the same region (read no. 4052). EMBL accession no. Z92540, bases 13000–131140. Sequence starts, CCTGCACGGCTCCCGGGAGCTGGGAGAAA; sequence ends, GTGAGTGCGAGGGGCCAGGCGGAGGGCCA.

This Article

  1. Genome Res. 8: 562-566

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