Sequence Quality and Signal Strength as a Function of the Number of Cycles
| No. of cycles | Percent accuracy and no. of ambiguous bases in 100-base intervals | Useful data range (bases) | Relative signal strength | |||||
| 1–100 | 101–700 | 701–800 | G | A | T | C | ||
| 35 | 98 (1) | 100 (0) | 99 (1) | 900 | 361 | 269 | 260 | 357 |
| 45 | 99 (0) | 100 (0) | 98 (1) | 850 | 477 | 311 | 229 | 403 |
| 75 | 100 (0) | 100 (0) | 99 (2) | 850 | 814 | 568 | 348 | 670 |
| 99 | 100 (0) | 100 (0) | 96 (4) | 780 | 1123 | 778 | 444 | 918 |
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An amount of 50 ng of pGEM was sequenced with BigDye terminators with 35, 45, 75, or 99 cycles. The accuracy of each sequence is listed as percentage agreement with the known sequence. The number following the percentage value is the number of ambiguous bases (N) for each interval. The usable data range is the lengths of sequence that are accurate after minor human editing of the computer generated base calls.
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↵These Ns and errors are due to incomplete removal of residual dye terminators, obscuring sequencing data where signals are somewhat weak.
