A Homogeneous, Ligase-Mediated DNA Diagnostic Test

Table 1.

PCR Primer and Ligation Probe Sequences

Name Sequence(5‘ → 3‘)
CF508-p1 GTGCATAGCAGAGTACCTGAAACAGGAAGTA
CF508-p2 TGATCCATTCACAGTAGCTTACCCATAGAGG
CF508-A FAM-CATTAAAGAAAATATCAT
CF508-B p-TGGTGTTTCCTATGA-ROX
CF508-C p-CTTTGGTGTTTCCTA-TAMRA
BRCAI185-p1 AATGGATTTATCTGCTCTTCGCGTTGAAGA
BRCAI185-p2 TCATTTGCATAGGAGATAATCATAGGAATCCCAA
BRCAI185-A FAM-ATGCAGAAAATCTTAG
BRCAI185-B p-TGTCCCATCTGTCT-ROX
BRCAI185-C p-AGTGTCCCATCTGTC-TAMRA
BRCAI5382-p1 GGGTTGTGTTTGGTTTCTTTCAGCATGA
BRCAI5382-p2 AGTCTTACAAAATGAAGCGGCCCATCTCT
BRCAI5382-A FAM-AGCAAGAGAATCCC
BRCAI5382-B p-CAGGACAGAAAGATCT-ROX
BRCAI5382-C p-AGGACAGAAAGATCTTC-TAMRA
HFE63-p1 GCTCTGTCTCCAGGTTCACACTCTC
HFE63-p2 GATCCCACCCTTTCAGACTCTGACTC
HFE63-A FAM-GTTCGTGTTCTATGAT
HFE63-B p-CATGAGAGTCGCC-ROX
HFE63-C p-GATGAGAGTCGCC-TAMRA
THAL-p1 CATGTGGAGACAGAGAAGACTCTTGGG
THAL-p2 GCAGCTCACTCAGTGTGGCAAAGG
THAL-A FAM-TCTACCCTTGGACC
THAL-B p-CAGAGGTTCTTTGAGT-ROX
THAL-C p-TAGAGGTTCTTTGAGTC-TAMRA
NAT-p1 GGGTGGTGTCTCCAGGTCAATCAAC
NAT-p2 CTGCCACATCTGGGAGGAGCTTC
NAT282-A FAM-TTAGGAGGGTATTTTTA
NAT282-B p-CATCCCTCCAGTTAA-TAMRA
NAT282-C p-TATCCCTCCAGTTAAC-ROX
NAT341-A FAM-ATTCCTGCCGTCA
NAT341-B p-ATGGTCACCTGCAG-TAMRA
NAT341-C p-GTGGTCACCTGCAG-ROX
D1S8-p1 CCACCCTTCCCACCAAACTACTCAC
D1S8-p2 CTTCTACCAATACCACCCTGTCACGTC
D1S8-A FAM-TCCAACCCTAGCC
D1S8-B p-GATACGGGAACGAC-ROX
D1S8-C p-AATACGGGAACGAC-TAMRA
s14102-p1 TAGCTGTCCATGCAGCGTGATTCACCA
s14102-p2 CTCTCCATCCTCTCTCCTCTCACACATTAGTTTACAC
s14102-A FAM-TGGCTTAGTGGTTTC
s14102-B p-ATTGTTTTATTTTTGTAAA-ROX
s14102-C p-GTTGTTTTATTTTTGTAAA-TAMRA
RDS-p1 TGCTATCCTGTGTCTTCAACTCGCT
RDS-p2 GGTAGTACTTCATGCCGTTCTTGAG
RDS-A FAM-CCTGGCTATCTGTGT
RDS-B p-CCTCTTCAACATCAT-ROX
RDS-C p-TCTCTTCAACATCAT-TAMRA
  • p1/p2 are the forward and reverse PCR primers.

  • FAM, ROX, and TAMRA are fluorescent donor and acceptor dyes; (p) 5‘-phosphorylation.

This Article

  1. Genome Res. 8: 549-556

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