Figure 1.
(A) Analysis of a 705-bp region (codons 482–715, E. coli numbering) of the Mycobacterium rpoB gene (1164 amino acids). (B) Region encompassing the 81-bp domain, described previously as having all of the mutations that correlate with the decreased sensitivity to rifampin (Musser 1995). Arrows indicate locations of PCR amplification primers and all of the primers used for dideoxynucleotide sequencing (see Methods for sequence). (C) Codon positions within the 81-bp region, containing mutations in 41 of the 44 RIF-resistant M. tuberculosis mutants. The frequency and type of mutation observed at each codon is presented.
