Figure 3.
Electropherograph showing the separation of a 157-bp PCR fragment of the HOXD gene in a 8% native polyacrylamide gel by use of a DNA sequencing instrument (ALF Express, Pharmacia Biotech).HOXD was amplified from human (H), pig (P), mouse (M), one pig/mouse SCH clone containing the pig HOXD gene (SCHP+), and one clone negative for the pig HOXD gene (SCHP−). Only the human product was amplified by use of fluorescently labeled primers and this product was added to the other amplification products prior to the denaturation/renaturation treatment. As a consequence, only human homoduplexes and human/mouse and human/pig xenoduplexes were visualized.
