Analysis of EST-Driven Gene Annotation in Human Genomic Sequence

Table 2.

Linking of ESTs by cDNA Clone ID

Data set Class Det. both Missed 5‘ Missed 3‘ No 5‘ No 3‘ Pol. unk. Efficacy
AS 95L 6 17 13 10 18 36 17
95S 6 17 13 10 17 37 18
90L 23 8 9 10 22 28 58
90S 18 8 8 10 28 27 53
80L 16 10 8 10 28 29 48
80S 10 14 10 11 28 27 31
70L 11 14 10 11 28 26 31
70S 10 15 11 11 26 27 27
WG 95L 7 20 14 11 19 30 18
95S 7 19 14 11 18 31 18
90L 27 7 9 10 23 23 63
90S 24 6 7 10 31 23 66
80L 20 8 7 10 30 25 59
80S 13 12 9 10 31 25 39
70L 14 11 9 10 32 24 40
70S 12 13 10 11 29 25 34
  • Linking results for cDNA clones derived from all ESTs aligning with entries in the two large test sets. For all columns except the last, values are the percentage of cDNA clones contributing ESTs to the specified stringency class for which the indicated linking result was obtained. Column headings are as follows: (Det. both) ESTs from both ends of the clone fall into the specified stringency class; (Missed 5‘) the ESTs from both ends of the clone are present in dbEST, but only the 3‘ EST falls into the specified stringency class; (Missed 3‘) the ESTs from both ends of the clone are present in dbEST, but only the 5‘ EST falls into the specified stringency class; (No 5‘) the 5‘ EST from the clone is not present in dbEST; (No 3‘) the 3‘ EST from the clone is not present in dbEST; (Pol. unk.) it was not possible to determine the polarity of ESTs from the clone; (Efficacy) the percentage of clones having ESTs from both ends in dbEST for which both ends were detected in the given stringency class.

This Article

  1. Genome Res. 8: 362-376

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