Minimum tile set for chromosome 4. L. major Friedlin cosmid clones were fingerprinted, as detailed in Methods, prior to contig assembly by CONTASP and FPC v. 2.8.2 software. Subcontigs shown to contain chromosome 4 marker loci were joined by (1) the application of additional software packages: MAPSUB (identified loose fingerprint matches), EXTEND (identified potential end–end joins), and (2) hybridization data (assigned and joined subcontigs), to yield an integrated map. The contig display from FPC formed the basis of this diagram. All clones that constitute the contig, including those that have been buried, are shown as horizontal lines. DNA hybridization probes are shown along the top (see Methods for probe descriptions). A minimum tile set (•) was chosen for DNA sequencing purposes. The scale bar represents 26 consensus bands (∼35 kb).
