A Physical Map of the Leishmania majorFriedlin Genome
Abstract
An extensive physical map of the Leishmania majorFriedlin genome has been assembled by the combination of fingerprint analysis of a shuttle vector cosmid library and probe hybridization. The integrated data obtained for 9004 fingerprinted clones and 974 probes have placed 91.2% of the 33.58-Mb genome into contigs representing each of the 36 chromosomes. This first-generation map has already provided a suitable framework for both high-throughput DNA sequencing and functional studies of the L. major parasite.
Footnotes
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↵1 Corresponding author.
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E-MAIL a.ivens{at}ic.ac.uk; FAX 44-171-2250960.
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- Received September 23, 1997.
- Accepted January 14, 1998.
- Cold Spring Harbor Laboratory Press
