The C. parvum karyotype. (a) the Saccaromyces cerevisiae (Y) and C. parvum (C) karyotypes resolved by PFGE. For S. cerevisiae sizes are given in Mb; for C. parvum, band 1 = chromosome I (1.04 Mb), band 2 = chromosome II (1.08Mb), band 3 = chromosomes III, IV, and V (1.24Mb), band 4 = chromosome VI (1.44Mb), and band 5 = chromosomes VII and VIII (1.54 Mb). (b) assignment of markers to individualCryptosporidium chromosomes following restriction digestion. Total Cryptosporidium DNA was digested by NotI (N) orSfiI (S), separated by PFGE (left panel) then blotted and probed. Marker cr1046 lies on a NotI fragment of 1.1 Mb and a SfiI fragment of 0.87 Mb, characteristic of chromosome III. Marker cr273 lies on a NotI fragment of 0.37 Mb and aSfiI fragment of 1.08 Mb, characteristic of chromosome V. Marker cr1011 lies on a NotI fragment of 0.77 Mb and aSfiI fragment of 1.08 Mb, also characteristic of chromosome V (see Caccio et al. 1998).
