Figure 1.
Classes of arrayed oligonucleotides. Each position is interrogated with 10 separate 25-mer oligonucleotides, 5 (two wild-type and 3-base substitution) each for sense and antisense strands. Substitution probes contain each of the four-nucleotide substitutions 13 bases from the 3′ end of the oligonucleotide (one of these will represent the wild-type sequence). A redundant set of wild-type perfect match probes are tiled in the lower portion of the array.
