Figure 2.
CeRep25B is responsible for the eP97 RFLP. (A) Southern blot of genomic DNA from N2 and RC301 strains probed with cosmid W10B10. TheeP97 polymorphism is indicated with an arrow. (B) PCR amplification of genomic DNA from a variety of C. elegansisolates. Genomic DNA, which was used as the template, is indicated. (See Methods for strain and PCR details.) The three panels are aligned such that relative fragment size is not maintained. Arrows indicate positions of wild-type and RC301 PCR fragments for unc-45intron 3.
