Multivariate Analysis of Factors Influencing Repeat Expansion Detection

Table 3.

RED Protocol

Ligation temp. 77°C
Ligation time 20 sec
Denaturation temp. 94°C
Denaturation time 10 sec
Cycle number 500
Enzyme 15 units
DNA 1.0 μg
Oligonucleotide 100 ng

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  1. Genome Res. 8: 1085-1094

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