Table 2.
PLS Regression Analysis
| X variables | Results of Selection | Results of first (1) and second (2) optimization | 1 and 2 (180 nt) | 1 and 2 (360 nt) | |||||
| 180 nt | 360 nt | 1 (180 nt) | 1 (360 nt) | 2 (180 nt) | 2 (360 nt) | ||||
| Ligation temp. | −0.45 ± 0.16 | −0.23 ± 0.11 | −0.64 ± 0.02 | −0.41 ± 0.06 | NS | NS | −0.22 ± 0.04 | −0.18 ± 0.02 | |
| Cycles | 0.35 ± 0.16 | 0.28 ± 0.11 | 0.17 ± 0.05 | 0.34 ± 0.07 | 0.077 ± 0.04 | 0.046 ± 0.02 | |||
| Enzyme conc. | NS | 0.24 ± 0.11 | 0.20 ± 0.07 | 0.32 ± 0.07 | 0.10 ± 0.09 | NS | 0.088 ± 0.04 | ||
| DNA conc. | 0.22 ± 0.16 | NS | NS | NS | |||||
| Oligo conc. | NS | NS | |||||||
| Ligation time | NS | NS | 0.19 ± 0.05 | 0.22 ± 0.06 | −0.078 ± 0.04 | −0.12 ± 0.02 | |||
| Temp. (2) | 0.32 ± 0.05 | 0.22 ± 0.16 | −0.24 ± 0.07 | −0.25 ± 0.08 | −0.13 ± 0.02 | −0.081 ± 0.03 | |||
| Cycles (2) | −0.02 ± 0.01 | −0.31 ± 0.05 | 0.10 ± 0.03 | −0.16 ± 0.03 | |||||
| Enzyme conc. (2) | NS | 0.19 ± 0.09 | 0.12 ± 0.10 | −0.14 ± 0.06 | 0.15 ± 0.03 | ||||
| DNA conc. (2) | −0.190 ± 0.08 | −0.076 ± 0.04 | |||||||
| Time (2) | 0.16 ± 0.04 | 0.14 ± 0.05 | −0.062 ± 0.02 | −0.039 ± 0.01 | |||||
| Temp. × cycles | 0.09 ± 0.04 | −0.11 ± 0.07 | −0.10 ± 0.08 | NS | 0.047 ± 0.03 | ||||
| Temp. × enzyme conc. | NS | NS | NS | −0.072 ± 0.03 | |||||
| Temp. × DNA conc. | NS | NS | |||||||
| Temp. × time | −0.11 ± 0.06 | NS | −0.12 ± 0.02 | −0.12 ± 0.02 | |||||
| Cycles × enxyme conc. | 0.16 ± 0.10 | 0.090 ± 0.05 | |||||||
| Cycles × DNA conc. | −0.08 ± 0.04 | ||||||||
| Cycles × time | 0.089 ± 0.05 | ||||||||
| Enzyme conc. × time | NS | −0.14 ± 0.05 | NS | ||||||
-
Regression coefficients are given with the confidence limits atP = 0.05. (nt) = Nucleotides.
