Table 1.
Computer-Assisted Value Selection for RED Optimization
| Levels | Selection | Optimization 1–180 | Optimization 1–360 | Optimization 2 180/360 | |||||||||
| − | + | −α | 0 | +α | −α | 0 | +α | −α | − | 0 | + | +α | |
| Ligation temp. (°C) | 75 | 85 | 72 | 80 | 88 | 72 | 80 | 88 | 57 | 60 | 65 | 70 | 73 |
| Ligation time (sec) | 20 | 60 | 40 | 40 | 3 | 10 | 20 | 30 | 37 | ||||
| Number of cycles | 200 | 800 | 50 | 500 | 950 | 50 | 500 | 950 | 500 | ||||
| Enzyme (units) | 5 | 25 | 15 | 1 | 15 | 29 | 7 | 10 | 15 | 20 | 23 | ||
| DNA (μg) | 0.5 | 2.0 | 0.2 | 1.25 | 2.3 | 1.25 | 1.25 | ||||||
| Oligonucleotide (ng) | 50 | 150 | 100 | 100 | 100 | ||||||||
