Multiple alignments of the identified protein-kinase families. Arrows indicate β-strands in the mouse MAPK structure (PDB accession no.1P38) (Wang et al. 1997). Broken arrows indicate that the respective strand includes gaps introduced by alignment to the protein kinase families described here. All sequences are listed with their GenBank identifiers followed by their systematic ORF names or a short mnemonic name. Numbers preceding, within, or following the aligned regions refer to the number of amino acid residues elided. Question marks indicate unknown peptide lengths in ORFs conceptually translated from EST sequences. Consensus key: Uppercase letters indicate conserved residues by the single-letter amino acid code, lowercase letters indicate conserved classes of amino acids that are highlighted as follows: [yellow (h)] hydrophobic residues (A,C,F,I,L,M,V,W,Y); [blue (p)] polar residues (C,D,E,H,K,N,Q,R,S,T); [red (c)] charged residues (D,E,H,K,R); [green (u)] tiny residues (A,C,G,S); (magenta) other conserved residues, as annotated in the consensus sequence [(s) small residues (A,C,D,N,G,P,S,T,V); (b) bulky residues (E,F,I,K,L,M,Q,R,W,Y); (a) aromatic residues (F,H,W,Y); (l) aliphatic residues (I,L,V), (+) positively charged residues (H,K,R), (−) negatively charged residues (D,E)]. The est_CElegans protein sequence in B is an artificial composite of ORFs from two EST sequences (gi2378893 and gi1121991). The MJ1211 sequence shown here actually differs from gi1591840 by the selection of an upstream start codon, adding 95 amino-terminal residues, including counterparts to the conserved regions I and II of the ePKs. The GenBank identifier of est_human inC (gi2357825) refers to a nucleotide sequence; a conceptual translation of one ORF is shown here.
