Physical maps of the USH1C region. (A) Somatic cell hybrids were used initially to bin markers believed to map betweenD11S889 and D11S861. The heavy lines represent the approximate extent of chromosome 11 from pter contained in each hybrid cell line. The order within each bin is based on the YAC map below. The placement of markers D11S899, D11S419, and D11S861 on the hybrid panel was determined by Fantes et al. (1995). These markers are for reference only and are not present within the clone contig. (B) YAC contig of USH1C critical and flanking regions. The contig was assembled by SEGMAP using both STS content andAlu–PCR hybridization data. YAC names and sizes (kb) are shown to the left of each clone and in parenthesis, respectively. For some clones (boxed), the cytogenetic position determined by FISH is indicated above the horizontal line representing the YAC. Markers contained within the contig are shown above the contiguous line that represents the genomic map. Markers with the prefix y47, y3, or ySJ are Alu–PCR probes generated from the named YAC clone using one of three Alu primers as described (Qin et al. 1996). Marker names prefixed with yRP and ending with RR, ER, or RL are end probes generated from the named YAC clone. The only link between the LDHA/SAA1-containing clones and the remainder of the contig is through the mega-YAC 804C12 determined byAlu–PCR hybridization. The proximity of these clones with the rest of the contig is consistent with other YAC contigs in this region (Sellar et al. 1994; Ayyagari et al. 1996). The ends of a PAC clone (59M18) were used to show a second link between the TPH YAC clones and KCNC1/S1888/MYOD1 YAC clones.
