Gene Discovery by EST Sequencing in Toxoplasma gondiiReveals Sequences Restricted to the Apicomplexa

Table 1.

Summary of Most Abundant ESTs by Library as Determined by Cluster Analysis

Rank RH tachyzoite cDNA library ME49 tachyzoite cDNA library
dbEST ID no. no. gene/description dbEST ID no. no. gene/description
1 465887 107 GRA1 p24 dense granule protein 571382 42 SAG1 p30 surface antigen
2 466589 69 GRA2 p28 dense granule protein 571076 32 GRA2 p28 dense granule protein
3 465733 68 GRA6 p32 dense granule protein 571569 29 GRA1 p24 dense granule protein
4 465767 64 SAG1 p30 surface antigen 571208 28 unknown (similar to RH 467174)
5 465877 58 GRA5 p21 dense granule protein 571108 27 SAG2 p22 surface protein
6 466942 53 SAG2 p22 surface antigen 571574 17 GRA5 p21 dense granule protein
7 467174 34 unknown (similar to ME49 571208) 571542 15 unknown (similar to RH 467213
8 465857 29 S8 ribosomal protein 571282 15 unknown (similar to RH 466613)
9 466627 28 GRA3 p30 dense granule antigen 571237 11 unknown (similar to RH 466242)
10 466406 26 unknown (similar to ME49 574388) 571154 9 GRA6 p32 dense granule protein
11 465750 22 unknown (similar to ME49 571377) 571377 9 unknown (similar to RH 465750)
12 466392 21 35-kD toxoplasma polypeptide 571392 9 unknown (similar to Schizosaccharomyces pombe7.67961)
13 467170 19 unknown (similar to ME49 604952) 571332 8 S2 ribosomal protein
14 465671 18 S30 ribosomal protein 571072 8 L18a ribosomal protein
15 466619 17 S7–S8 ribosomal protein 574528 8 L22 ribosomal protein
  • Total number of ESTs for the RH tachyzoite cDNA library was 5359; total number of ESTs for the ME49 tachyzoite cDNA library was 1806.

  • EST frequency is fivefold more abundant in RH.

  • EST frequency is fivefold more abundant in ME49.

This Article

  1. Genome Res. 8: 18-28

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