RNA blot analysis of mRNA from mouse tissues probed with exon 2 ofDfy. (Lane 1) 2 μg of bone marrow mRNA; (lane2) 5 μg of spleen mRNA; (lane 3) 5 μg of liver mRNA; (lane 4) 5 μg of lung mRNA; (lane 5) 10 μg of kidney mRNA; (lane 6) 10 μg of heart mRNA; (lane 7) 5 μg of skeletal muscle mRNA; (lane 8) 1 μg of brain mRNA. Actin probe was used to test the integrity of the mRNA fraction and to determine the relative abundance of poly(A)+RNA. RNAs were resolved on a 1.5% denaturing agarose gel hybridized and autoradiographed for 5 days. The relative amount of mRNA was determined with the Eagle Eye II Still Video System (Stratagene). The Duffy-like mRNA/actin mRNA ratios were as follows: (lane 1) 1.80; (lane 2) 2.30; (lane 3) 0.78; (lane 4) −; (lane 5) −; (lane 6) 0.09; (lane 7) 0.09; (lane 8) 10.72.
