Figure 3.
The presence within the library of seven known CGIs was shown by PCR amplification from pooled library stocks (C). Product was also amplified from pig DNA (P) as a positive control, and a no-DNA negative control (−) was always included. Primers used were arachidonate 12-lipoxygenase, follistatin, G protein α subunit, heat shock protein 70, and three anonymous pig CGIs. Primers for a CpG poor region of interferon α were also used to show the absence of this DNA from the library. (M) Marker.
