Characterization of Short Tandem Repeats from Thirty-One Human Telomeres
- Marjorie Rosenberg1,
- Lester Hui2,
- Junli Ma2,
- Harris C. Nusbaum2,
- Kevin Clark3,
- Louise Robinson3,
- Laura Dziadzio1,
- Pamela M. Swain4,
- Tim Keith4,
- Thomas J. Hudson2,
- Leslie G. Biesecker1, and
- Jonathan Flint3,5
- 1National Human Genome Research Institute, Laboratory for Genetic Disease Research, National Institutes of Health, Bethesda, Maryland 20892; 2Whitehead Institute for Biomedial Research, Cambridge, Massachusetts 02142; 3Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, OX3 9DS, UK; 4Genome Therapeutics, Waltham, Massachusetts 02154
Abstract
Completion of genetic and physical maps requires markers from the ends (telomeres) of every human chromosome. We have searched for short tandem repeats (microsatellites) in cosmid and P1 clones and generated 661 sequence-tagged sites (STS) from the terminal 300 kb of 31 human chromosome ends. PCR assays were successfully designed for 58 microsatellites and mapped both genetically and on radiation hybrids (RHs) to confirm their telomeric location. Sequence analysis revealed marked variation in sequence composition, consistent with the hypothesis that even very highly GC-rich chromosome bands (the T bands) are not homogenous. The STSs that we have generated will be a necessary resource for the construction of physical maps of these complex regions of the genome.
[Information about the microsatellites is available electronically at http://www.cshl.org/gr and sequence has been deposited in the Genome Database (GDB).]
Footnotes
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↵5 Corresponding author.
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E-MAIL jf{at}worf.molbiol.ox.ac.uk.; FAX 44 1865 222500.
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- Received May 22, 1997.
- Accepted July 24, 1997.
- Cold Spring Harbor Laboratory Press
