(A,B) Agarose gel electrophoresis of PCR products (513 bp) obtained from RT–PCR using total RNA from embryos or fetuses of different normal (A)- or double (B)-muscled Belgian Blue bovine developmental stages. (M) Markers (1-kb ladder from GIBCO BRL). Different embryonic or fetal ages are indicated in corresponding lanes. The locations of the primers used to amplify 513-bp partial cDNA are from amino acid 202 to 208 (sense primer) and from amino acid 365 to 371 (antisense primer). (See Methods for primer sequence.) This 513-bp partial cDNA contains the 11-bp deletion observed in double-muscled Belgian Blue cattle. (C) Expression of myostatin in different adult bovine muscles. Fifteen micrograms of total RNA was electrophoresed on formaldehyde–agarose gel, blotted onto nylon membrane, and probed with bovine myostatin cDNA. (Lane 1) M. gastrocnemius; (lane 2) M. psoas major; (lane 3) M. longissimus dorsi; (lane 4) M. biceps femoris; (lane 5) M. diaphragm; (lane 6) M. semimembranosus; (lane 7) M. flexor digitorum longus; (lane 8) M. vastus medialis; (lane 9) M. vastus lateralis; (lane 10) heart; (lane 11) M. cutaneus trunci; (lane 12) M. semitendinosus; (lane 13) M. semitendinosus (normal, 260 day); (lane 14) M. semitendinosus (double-muscle, 260 day).
