Sequence analysis of normal- and double-muscled bovine myostatin. Sequencing was performed on three independent normal- and double-muscled alleles, and one representative sequence of both alleles is shown. (A) Amino acid sequence comparisons of mouse (MMYO.PRO) and bovine Myostatin (BMYO.PRO) proteins. Nonconserved amino acids are indicated by solid bars. The consensus amino acid sequence is shown at the top. The proteolytic processing site is underlined. (B) The deletion mutation is detected by fluorometric sequencing of myostatin cDNA from normal- and double-muscled cattle. The sequence of the double-muscled allele is shown above that of the normal allele (Control), and the position where 11 bp is deleted in the mutant allele is indicated by an arrow. The large bracket in the normal allele sequence denotes the region that is deleted in the double-muscled allele. (C) The amino acid sequence of Myostatin in normal cattle is shown above the predicted amino acid sequence of Myostatin in double-muscled cattle. The premature stop codon at amino acid position 288 in the double-muscle allele is indicated by an asterisk (*). (D) The predicted amino acid sequence of Myostatin in normal cattle is shown below that of the Piedmontese breed in the vicinity of the transition mutation. The altered residue in the Piedmontese allele is underlined. Asterisks (*) indicate two of the nine conserved cysteine residues in exon 3 of the normal bmyostatin allele.
