Figure 2.
The relationship between the number of doublings and the mutant fraction of all mutants created by Pfu. The mutant fractions from three independent experiments were estimated by comparing the total area of all mutant peaks with the area of an internal standard. A linear regression analysis was applied to all data points. By dividing the estimated slope by the length of the low-temperature melting domain of our DNA fragment (96 bp), we calculated an error rate of 6.5 ± 3.1 × 10−7 mutants per base pair per doubling (95% confidence interval).
