Analysis by CDCE of the purified homoduplex mutant fractions after 14 and 34 doublings using Pfu DNA polymerase. This CDCE was run using a 5% polyacrylamide gel in a 35-cm capillary with a 15-cm heated zone at a temperature of 70.6°C with a 5-μA current. Many small peaks after 14 doublings may be seen to increase significantly after 34 doublings. Of the major peaks for which the mutant sequences were determined, peak A is an AT → CG transversion at bp 10071 of human mitochondrial DNA; peak B is an AT → GC transition at bp 10108; peak C is an AT deletion occurring in a run of six consecutive AT base pairs at positions 10048–10053; peak D is an AT → TA transversion at bp 10071; and peak E is a GC → TA transversion at bp 10070.
