A >3-Mb contig of overlapping YAC, PAC, and cosmid clones. STS andAlu–PCR product screening reagents are listed above the black line, and positions are identified by vertical lines. Genetic markers are indicated in blue, genes in green, STSs in red, and bacteriophageAlu products in purple. (Phage cluster) Corresponding phage clone-derived Alu–PCR products listed in Table 1. (*ESTs)D11S951E, D11S1956E, and WI-12191. (a1)Alu–PCR products obtained using 263 primers; (a2) PCR products obtained using S/J primers; (a3) PCR products obtained using 278 primers (see Methods). Clones are represented by horizontal lines: red lines represent PAC clones, blue lines represent cosmid clones, and black lines represent YAC clones. Corresponding STS/Alu–PCR content is shown by vertical lines. PAC size is estimated at 125 kb and cosmid size at 40 kb. An open circle identifies a probable internal deletion. Parentheses indicate unstable clones (i.e., strains that have different insert sizes in two separate isolations). To simplify the diagram, not all of the screening reagents/clones used for the establishment of the contig are depicted if the information was redundant. Table 1 lists the screening materials and clones used in the development of the contig depicted here and can be accessed athttp://genome.wustl.edu/gerhard/gerhard.html orhttp://www.cshl.or/gr.
