Sequence Mapping by Electronic PCR

Table 2.

Numbers of Matches Found with Various Types of Sequences and STSs

Sequence type/ STS type Sequences searched Sequences with matching sites (%) Sequences with sites/1000 STSs
Genomic 426
 random 24 (6) 6.8
 microsatellite 31 (7) 5.9
 transcript 133 (31) 8.3
mRNA 16,691
 random 3 (<0.01) 0.85
 microsatellite 7 (<0.01) 1.3
 transcript 3,553 (21) 221
ESTs, 5′ reads 248,589
 random 18 (<0.01) 5.1
 microsatellite 4 (<0.01) 0.76
 transcript 8,591 (3) 534
ESTs, 3′ reads 211,942
 random 16 (<0.01) 4.5
 microsatellite 3 (<0.01) 0.57
 transcript 49,617 (23) 3,082
  • The random STS set was derived from The Whitehead physical map (Hudson et al. 1995) and consisted of 3519 markers that had been developed from random DNA fragments. Microsatellite STSs were the 5264 markers from the Généthon genetic map (Dib et al. 1996). Transcript-based STSs were a nonredundant set of 16,294 markers from the international RH consortium transcript map (Schuler et al. 1996). The genomic sequence set is the same one described in Table 1. The mRNA set consisted of all human, nonmitochondrial mRNA sequences from the GenBank PRI (primate) division. The two human EST sets were taken from the EST division, making use of information present in dbEST for the end of the clone insert that was read.

This Article

  1. Genome Res. 7: 541-550

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