Overlapping PCR for Bidirectional PCR Amplification of Specific Alleles: A Rapid One-Tube Method for Simultaneously Differentiating Homozygotes and Heterozygotes

Table 2.

List of Primers in the Prospective Study

 Name         Sequence        WT/M  T m (°C)   Tail     Yield  Specificity
graphic
  • See Table 1 footnote for details on primer sequence, etc.

  • The sequence of the FIX gene was numbered on the basis of Yoshitake et al. (1985).

  • The P and Q primers for FV (opp) were the same as used in FV (see Table 1).

  • The sequence of the D1 gene was submitted to GenBank under accession no. X55760.Gb_Pr.

This Article

  1. Genome Res. 7: 389-398

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