Bi-PASA reactions for the COMT and FV genes: Interactions among the primers. Two-, three-, and four-primer combinations for the WT/WT (lanes 1–6), M/M (lanes 7–12), and WT/M (lanes 13–18) templates were amplified to observe the efficiency of the primers. (M) Size standard (120 ng of φX174/HaeIII). (A) COMT gene Bi-PASA reaction. The inner primers used were A2 and B3 (see Table 1 for primer specifications). The primer concentrations were 0.05 μmfor P, Q, and B, and 0.1 μm for A. The annealing temperature was 65°C, and 5% DMSO was added to the reaction. (B) FV Bi-PASA reaction. The inner primers used were A11 and B11 (see Table 1). The primer concentrations were 0.1 μmfor P, B, and A, and 0.05 μm for Q. The annealing temperature was 55°C.
