Kinetics of Bi-PASA. Identical [α-32P]dATP-labeled Bi-PASA reactions were removed from the thermocycler every three cycles, and the yield for each segment was quantified with a PhosphorImager. Potentially, 135, 87, and 214 adenine nucleotides can be labeled by [α-32P]dATP in the PB, AQ, and PQ segments, respectively. (A) All four primers were used for WT/M, WT/WT, and M/M samples. The yields for potential segments were greater in the WT/WT and M/M samples, indicating that the type of genomic template used can affect the yields. (B) The three two-primer combinations for the WT/M sample were performed. A greater yield was observed for the three segments individually as compared to the WT/M sample in A, indicating that interactions among the primers can influence the yield in a Bi-PASA reaction.
