Figure 8.
Single nucleotide extension of unpurified BRCA1 PCR product.BRCA1 PCR product was amplified with primers F320 and R480 as described in Methods. Following the primer extension assay, an aliquot of unextended primers F320 and R480 was added to the mixture at a concentration of 0.2 μm to provide a mass reference for their single-base extension products. Measured mass differences between primers and their expected extension products differed from calculated values by a maximum of 0.14 dalton.
