Single-Nucleotide Polymorphism Identification Assays Using a Thermostable DNA Polymerase and Delayed Extraction MALDI-TOF Mass Spectrometry

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Figure 7.
Figure 7.

Single nucleotide extension with single and double-stranded target. The single nucleotide extension assay was conducted with ThermoSequenase as described in Methods in the presence of 10 μm ddNPTs. (A) Single-stranded target; (B) Target with 10% excess of complementary sequence; (C) Negative enzyme control.

This Article

  1. Genome Res. 7: 378-388

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