Single-Nucleotide Polymorphism Identification Assays Using a Thermostable DNA Polymerase and Delayed Extraction MALDI-TOF Mass Spectrometry

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Figure 6.
Figure 6.

Primer extension assay with heterozygous sample. The SNP assay was conducted with ThermoSequenase as described in Methods in the presence of 10 μm ddNTPs and 0.4 μm each of target sequences with C (wild-type) and T (mutant) at polymorphic site. (A) Negative enzyme control; (B) Plus ThermoSequenase.

This Article

  1. Genome Res. 7: 378-388

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