Single-Nucleotide Polymorphism Identification Assays Using a Thermostable DNA Polymerase and Delayed Extraction MALDI-TOF Mass Spectrometry

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Figure 2.
Figure 2.

Comparison of enzymes in primer extension. Primer extension product was produced in 25 thermal cycles. (A) negative enzyme control; (B) ThermoSequenase; (C) AmpiTaq DNA polymerase; (D) Exo− Pfu DNA polymerase; (E) exo+ Pfu DNA polymerase. Conditions were as described in Methods and in Fig. 1.

This Article

  1. Genome Res. 7: 378-388

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