Single-Nucleotide Polymorphism Identification Assays Using a Thermostable DNA Polymerase and Delayed Extraction MALDI-TOF Mass Spectrometry

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

Figure 1.
Figure 1.

Model system for single nucleotide polymorphism detection. SNP primer was annealed as indicated to the test sequence containing a polymorphic site at the underlined position. In the presence of a DNA polymerase and four ddNTPs, the primer is extended by a single base complementary to the polymorphic site. When subjected to DE-MALDI-TOF, the difference in mass between unextended and extended primer indicates which bases were added.

This Article

  1. Genome Res. 7: 378-388

Preprint Server



Current Issue

  1. January 2026, 36 (1)
  1. Current Issue
  1. Alert me to new issues of Genome Research