Cloning, Characterization, and Copy Number of the Murine Survival Motor Neuron Gene: Homolog of the Spinal Muscular Atrophy-Determining Gene

For each of the STSs indicated, PCR amplifications were performed in triplicate using single-colony picks or DNA prepared from single colonies of the indicated clone. (▪) Positives; (□) negatives. BAC clones were analyzed by restriction mapping and Southern blot hybridization using a variety of probes (data not shown). TheSmn STS marker represents all Smn primer sets listed in Table 1. Naip exon 5 and D13Mit37 are represented multiple times in the region (data not shown) but listed only once for simplicity. (G.S.) Genome Systems; (R.G.) Research Genetics; (NA) not analyzed; (MIT D.B.) MIT database.