Physical maps of the human α/δ TCR locus. (a) Seventeen BAC clones, with sizes as determined by PFGE indicated, spanning the α/δ locus. The BAC clones were characterized in several different ways: restriction enzyme analysis (HindIII and EcoRI sites shown), by localization of TCR elements by hybridization (•), by STS mapping (▪), and by PFGE analysis. These results were compared to similar data obtained for overlapping YAC and cosmid clones. The relative order for most across the locus is known because of a cosmid map of high redundancy. The exact order of several probes could not be determined in several BAC clones not covered by the cosmid map. The gap between the two BAC contigs is indicated as is the deletion in BAC196. (b) The positions of the sequenced BAC, PAC, and cosmid clones are indicated. (c) End sequences obtained from each BAC clone were compared to the final sequence and their positions indicated. The complete names of the BAC and PAC clones presented are as follows: BAC10G10, BAC116B10, BAC129A3, BAC135H10, BAC196E6, BAC259E10, BAC274H3, BAC363D9, BAC378F4, BAC417G3, BAC480F2, BAC540C5, BAC628B12, BAC705G10, BAC810D3, BAC916C11, BAC956H7, PAC161M22, and PAC230I21.
