Cloning-Free PCR-Based Allele Replacement Methods

Table 2.

Primers Used in this Study

Name Sequence
Adaptamerb for K. lactis URA3 CATGGCAATTCCCGGGGATCGTGATTCTGGGTAGAAGATCG
Adaptamera for K. lactis URA3 CATGGTGGTCAGCTGGAATTCGATGATGTAGTTTCTGGTT
K. lactis internal 5‘ primer CTTGACGTTCGTTCGACTGATGAGC
K. lactis internal 3‘ primer GAGCAATGAACCCAATAACGAAATC
AdaptamerARfa1 forRFA1 AATTCCAGCTGACCACCATGATGAGCAGTGTTCAACTTTC
AdaptamerBRfa1 forRFA1 GATCCCCGGGAATTGCCATGTTAAGCTAACAAAGCCTTGG
AdaptamerA1 forRFA1 AATTCCAGCTGACCACCATGTATTCAATGTCAACTTCTTG
AdaptamerA21 forRFA1 AATTCCAGCTGACCACCATGCAATCAAAGAGGTGATGG
AdaptamerA61 forRFA1 AATTCCAGCTGACCACCATGAAGAGTTTCCTACAAGGGAG
AdaptamerA81 forRFA1 AATTCCAGCTGACCACCATGAACGTTTGGACTATCAAAGC
AdaptamerAKar1 forKAR1 AATTCCAGCTGACCACCATGATGAATGTAACTTCTCCAAA
AdaptamerBKar1 forKAR1 GATCCCCGGGAATTGCCATGTTAAAACCTATAATACACAT
AdaptamerAmut forSGS1 AATTCCAGCTGACCACCATGGATCTCTTTGCTATCAACTTC
AdaptamerBSgs1 forSGS1 GATCCCCGGGAATTGCCATGCTTTCTTCCTCTGTAGTGACC
Mutamer forsgs1 GCCAACAGGGGGTGGTAGATCTCTTTGCTATC
RFA1–2A CAGAGCATCCAAATGAAACC
RFA1–3B TTTGGATAATACCGAGGACG

This Article

  1. Genome Res. 7: 1174-1183

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