Cloning-Free PCR-Based Allele Replacement Methods

Table 1.

Efficiency of Allele Transfer

Position of the mutation from 5‘ end of homology (bp) Percent with the mutation in both repeats (no.)
 21st 30 (23)
 41st 53 (32)
 81st 96 (26)
101st 100 (20)
682nd 100 (25)
  • The numbers in parentheses indicate the total number of transformants analyzed.

This Article

  1. Genome Res. 7: 1174-1183

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