Use of adaptamers to fuse two fragments. The matched adaptamersA and a contain complementary sequence tags at their 5′ ends, as described in the text (indicated as A and a on the PCR products). The 3′ ends of each adaptamer are homologous to two different DNA sequences, respectively. Adaptamer A, in conjunction with adaptamer B, differentially tags one fragment at each end. The primer is designed to permit the PCR amplification of the other fragment, as shown. After amplification, the fragments are mixed and excess primer and adaptamer B are added for an additional PCR step. The complementary sequence tags in adaptamersA and a direct the fusion of the two fragments leading to a chimeric product.
