Products of a sample of rePCRs. Fifteen ORFs were reamplified using the 70-base activation domain rePCR primers. The sizes of these ORFs from the genome sequence are YBR139W (1527 bp), YBR186w (1611 bp), YCL038c (1587 bp), YCR028c (1539 bp), YDL238c (1470 bp), YDL207w (1617 bp), YDL197c (1578 bp), YDL189w (1612 bp), YDL178w (1593 bp), YDL170w (1587 bp), YDL160c (1521 bp), YDL159w (1548 bp), YDL156w (1569 bp), YDL146w (1476 bp), and YDL143w (1587 bp). The DNA size marker in theright lane has fragments of 23, 9.4, 6.6, 4.4, 2.3, 2.0, 1.4, 1.1, 0.9, and 0.6 kb. The rePCR products all migrate slightly slower than the predicted sizes of their ORFs because of the 70 bp of extra sequence at each end.
