A Streamlined Mutation Detection System: Multicolor Post-PCR Fluorescence Labeling and Single-Strand Conformational Polymorphism Analysis by Capillary Electrophoresis

Table 4.

Statistical Evaluation of Mobility Shift

No. Mobility shift No. Mobility shift
sense antisense sense antisense
gyrA hFIX exon 7
 1 −8.69 23.51 1 −11.62 −0.93
 2 −11.21 −2.92 2 4.53 2.49
 3 27.16 1.96 3 126.41 166.66
 4 0.18 11.18 4 −12.58 −2.08
 5 53.21 −5.91 5 133.61 143.92
 6 77.69 54.97
 7 47.90 56.54
 8 67.03 71.18 hFIX exon 8
 9 −50.63 −24.51
10 −3.80 −1.95 1 11.13 52.11
11 −12.08 0.99 2 3.56 2.00
12 −4.83 −1.95 3 −55.12 −3.00
13 −3.80 14.11 4 15.43 32.93
14 −0.81 0.01 5 −41.01 −57.68
15 −10.05 −2.89 6 −35.14 −57.34
16 1.22 4.09
17 2.20 4.02
18 72.58 79.07
19 78.01 75.30
20 93.44 79.23
21 28.89 0.99
22 8.15 12.37
23 1.19 3.03

  • ↵Mobility shift is defined as a difference between data point of mutant peak and averaged data point of normal peak. The standard deviations of normal DNAs are gyrA sense strand, 0.40; gyrA antisense strand, 0.01; hFIX exon 7 sense strand, 0.46; hFIX exon 7 antisense strand, 0.10; hFIX exon 8 sense strand, 0.40; hFIX exon 8 antisense strand, 0.63.

  • ↵False negative (within 3× s.d. of normal DNA)

  • ↵A minor peak was observed at 0.04, presumably attributable to an isoconformer.

This Article

  1. Genome Res. 7: 1094-1103

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