A Streamlined Mutation Detection System: Multicolor Post-PCR Fluorescence Labeling and Single-Strand Conformational Polymorphism Analysis by Capillary Electrophoresis

Table 3.

Mutated Sequences in Sample DNA

No. Mutation Position No. Mutation Position
gyrA hFIX exon 7
 1 T → G 250 1 T → C 30046
 2 C → T 251 2 G → A 30070
 3 G → C 253 3 T → G 30117
 4 G → A 262 4 T → C 30134
 5 G → C 262 5 AC deletion 30141
 6 A → C 263
 7 A → G 263
 8 A → T 263 hFIX exon 8
 9 TC → AT 250
10 TC → AA 250 1 C → T 30875
11 TC → AG 250 2 C → A 30973
12 TC → CT 250 3 C → A 31096
13 CA → TG 251 4 G → A 31119
14 CA → TT 251 5 G → T 31211
15 CA → AT 251 6 A → G 30972
16 CA → TC 251 C → T 31328
17 CA → AC 251
18 GA → AT 262
19 GA → TT 262
20 AA → TT 263
21 TTC → CTT 249
22 TCA → GGT 250
23 GAA → ACT 262

  • ↵Nucleotide number starting from the first nucleotide of the coding sequence of gyrA (Munakata et al. 1994) or proposed transcription initiation site of hFIX (Yoshitake et al. 1985).

  • ↵This sample carried a double mutation, at positions 30972 and 31328.

This Article

  1. Genome Res. 7: 1094-1103

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