Table 2.
Calibration of Peak Positions of Normal DNA
| Sense strand | Antisense strand | ||||||
| raw | calibrated using | raw | calibrated using | ||||
| M | M + N | M | M + N | ||||
| 6393 | 6393.00 | 6393.00 | 6557 | 6557.00 | 6557.00 | ||
| 6474 | 6389.01 | 6393.03 | 6638 | 6556.32 | 6557.02 | ||
| 6457 | 6392.16 | 6392.98 | 6623 | 6560.09 | 6557.02 | ||
| 6476 | 6391.79 | 6393.01 | 6641 | 6560.12 | 6557.02 | ||
| 6469 | 6389.53 | 6392.01 | 6635 | 6557.35 | 6557.01 | ||
| average | 6391.10 | 6392.81 | 6558.18 | 6557.01 | |||
| s.d. | 1.55 | 0.40 | 1.61 | 0.01 | |||
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Data points of peaks of R6G-labeled sense strand and R110-labeled antisense strand of PCR product of gyrA wild type examined by five successive runs.
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↵Raw data without calibration.
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↵Calibrated using size marker alone.
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↵Calibrated using size marker and TAMRA-labeled normal PCR products.
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↵Used as a template for calibration.
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↵Standard deviation.
