A Streamlined Mutation Detection System: Multicolor Post-PCR Fluorescence Labeling and Single-Strand Conformational Polymorphism Analysis by Capillary Electrophoresis

Table 1.

Primers Designed for Postlabeling

Target Primer Nucleotide sequence Amplicon size (bp)
gyrA sense 5′-ATTTAGGCATGACAAGTGAC
antisense 5′-GTTTGTTCGATCTCAGCTTT 592
hFIX exon 7 sense 5′-atTGCCTATTCCTGTAACCAGCA
antisense 5′-gttCTGCTACAGGTTGTGCCAAG 521
hFIX exon 8 sense 5′-attCTGTGTATGTGAAATACTGTTTGTGA
antisense 5′-gtTCCCCTCTAAAGAGAAAAAGCA 741
  • Primers are designed to have 5′-ATT or 5′-GTT. When necessary, extranucleotides (lowercase) are attached for postlabeling purposes.

This Article

  1. Genome Res. 7: 1094-1103

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