Segregation of large polymorphic restriction fragments containing 180-bp centromeric repeat arrays in a recombinant inbred mapping population. High-molecular-weight genomic DNA samples from five A. thaliana Columbia (Col)/Landsberg (La) RI lines were digested withNcoI and size-fractionated by pulsed-field gel electrophoresis. After electrophoresis, the DNA was transferred to a nylon membrane and hybridized with a radiolabeled 180-bp repeat probe. Dominant markers corresponding to fragments 14 and 15 segregated independently. The inheritance of the markers represented by fragments 15 and 16 is mutually exclusive as expected for allelic markers in RI populations (Lister and Dean 1993).
